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a Sequential isolation and N-glycan profiling of immunoglobulin G (IgG) and Tf from two human populations (Korcula and VIKING). HILIC-UHPLC-FLD ultra-high-performance liquid chromatography based on hydrophilic interactions with fluorescent detection. b Workflow for Tf purity assessment and N-glycan structures characterization by several complementary approaches. nanoLC-ESI-qTOF-MS nano-liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass <t>spectrometry;</t> MALDI-TOF-MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
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a Sequential isolation and N-glycan profiling of immunoglobulin G (IgG) and Tf from two human populations (Korcula and VIKING). HILIC-UHPLC-FLD ultra-high-performance liquid chromatography based on hydrophilic interactions with fluorescent detection. b Workflow for Tf purity assessment and N-glycan structures characterization by several complementary approaches. nanoLC-ESI-qTOF-MS nano-liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry; MALDI-TOF-MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Journal: Communications Biology

Article Title: Comparative analysis of transferrin and IgG N-glycosylation in two human populations

doi: 10.1038/s42003-023-04685-6

Figure Lengend Snippet: a Sequential isolation and N-glycan profiling of immunoglobulin G (IgG) and Tf from two human populations (Korcula and VIKING). HILIC-UHPLC-FLD ultra-high-performance liquid chromatography based on hydrophilic interactions with fluorescent detection. b Workflow for Tf purity assessment and N-glycan structures characterization by several complementary approaches. nanoLC-ESI-qTOF-MS nano-liquid chromatography coupled to electrospray ionization quadrupole time-of-flight mass spectrometry; MALDI-TOF-MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Article Snippet: Tandem mass spectrometry (MALDI-TOF/TOF-MS/MS) was performed via LIFT TM technology (Bruker Daltonics).

Techniques: Isolation, Glycoproteomics, Hydrophilic Interaction Liquid Chromatography, High Performance Liquid Chromatography, Liquid Chromatography, Mass Spectrometry